Plant pathogens can be detected and identified using ELISA and PCR
A. ELISA based detection is more useful for viral pathogen detection than bacterial pathogens
B. Fungal diseases are more amenable for ELISA based detection than plant viruses
C. Bacterial pathogens are more amenable for PCR based detection than plant viruses
D. Bacterial pathogens are more amenable for PCR based detection than fungal pathogens
E. Phytoplasma is more amenable for PCR based detection than ELISA
Choose the most appropriate answer from the options given below:
A. ELISA based detection is more useful for viral pathogen detection than bacterial pathogens
B. Fungal diseases are more amenable for ELISA based detection than plant viruses
C. Bacterial pathogens are more amenable for PCR based detection than plant viruses
D. Bacterial pathogens are more amenable for PCR based detection than fungal pathogens
E. Phytoplasma is more amenable for PCR based detection than ELISA
Choose the most appropriate answer from the options given below:
Show Hint
PCR = Detects DNA/RNA (Great for anything with low concentration like Phytoplasma).
- B and C only
- D and E only
- B and D only
- A and E only
The Correct Option is D
Solution and Explanation
The question asks to compare the efficiency and suitability of two major diagnostic tools: ELISA (Enzyme-Linked Immunosorbent Assay - protein-based) and PCR (Polymerase Chain Reaction - DNA/RNA-based) for different types of plant pathogens.
Step 2: Detailed Explanation:
• Statement A (ELISA for Viruses): Viruses have high protein-to-nucleic acid ratios (capsid proteins), making them excellent targets for antibody-based ELISA. Bacteria, being larger and more complex, are often better detected by nucleic acid methods. Thus, A is Correct.
• Statement E (Phytoplasma and PCR): Phytoplasmas are specialized bacteria that cannot be cultured. They are found in very low titers in the phloem. PCR is far more sensitive than ELISA for detecting these low-concentration, wall-less prokaryotes. Thus, E is Correct.
• Why others are incorrect:
- B: Viruses are actually the primary targets for ELISA, not fungi.
- C & D: PCR is highly efficient for all these pathogens; there isn't a definitive rule that makes bacteria "more amenable" than viruses or fungi in a way that excludes the others. Detection efficacy depends on specific primer design.
Final Answer:
The most universally accepted technical Suitabilities are A and E. Option (D) is correct.
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(A). Grow-out test & (I). Leachate discharge
(B). Tetrazolium test & (II). Seed coat permeability
(C). Electrical conductivity & (III). Seed purity
(D). Imbibition test & (IV). Seed viability
Choose the correct answer from the options given below: - Match List-I with List-II
List-I: Seed type & List-II: Examples
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(D). Hypogeal germination & (IV). Soybean (wild type)
Choose the correct answer from the options given below:
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