Question:
One of the methodologies listed below is NOT used for DNA fingerprinting :
One of the methodologies listed below is NOT used for DNA fingerprinting :
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DNA fingerprinting markers: RFLP, RAPD, SSR, AFLP, SNP. RT-PCR is used for RNA/gene expression analysis.
Updated On: Sep 15, 2026
- Restriction Fragment Length Polymorphism (RFLP)
- Simple Sequence Repeats (SSR)
- Reverse Transcription–Polymerase Chain Reaction
- Single Nucleotide Polymorphisms (SNPs)
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept:
DNA fingerprinting and profiling methods identify stable genomic sequence polymorphisms across individuals.
Step 2: Detailed Explanation:
RFLPs (hybridization-based restriction site polymorphism), SSRs/microsatellites (tandem repeat length polymorphisms), and SNPs (single base locus variations) are standard genomic DNA marker techniques used for DNA fingerprinting and genotyping.
Reverse Transcription-PCR (RT-PCR) is an expression profiling assay that converts mRNA into cDNA to quantify gene expression levels or detect RNA viruses, rather than assaying genomic DNA polymorphism for fingerprinting.
Step 3: Final Answer:
Hence, RT-PCR is NOT used for DNA fingerprinting.
DNA fingerprinting and profiling methods identify stable genomic sequence polymorphisms across individuals.
Step 2: Detailed Explanation:
RFLPs (hybridization-based restriction site polymorphism), SSRs/microsatellites (tandem repeat length polymorphisms), and SNPs (single base locus variations) are standard genomic DNA marker techniques used for DNA fingerprinting and genotyping.
Reverse Transcription-PCR (RT-PCR) is an expression profiling assay that converts mRNA into cDNA to quantify gene expression levels or detect RNA viruses, rather than assaying genomic DNA polymorphism for fingerprinting.
Step 3: Final Answer:
Hence, RT-PCR is NOT used for DNA fingerprinting.
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