Question

Match List I with List II 

 

• A. A-III, B-II, C-IV, D-I
• B. A-III, B-I, C-IV, D-II
• C. A-II, B-III, C-IV, D-I
• D. A-IV, B-III, C-II, D-I

Hint:

Dien's stain is a unique diagnostic stain exclusively used to identify Mycoplasma spp.
Remembering that C matches with IV instantly narrows down the possibilities to options (A) and (B), and pairing Acid-fast with Mycobacterium (B-I) confirms the correct option as (B).

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Bacterial staining techniques rely on chemical interactions between specialized dyes and unique target cellular structures (such as cell wall lipids, metachromatic granules, or specialized membranes) to visualize microorganisms under a microscope.

Step 2: Detailed Explanation:
Let us correlate each histochemical staining technique with its target bacterial group:
- A. Machiavello staining: This is a classical differential staining technique used specifically to visualize elementary bodies of obligate intracellular pathogens belonging to Chlamydia spp, staining them bright red against a blue background.
Thus, A matches with III.
- B. Acid-fast staining: This technique (such as the Ziehl-Neelsen stain) targets the high lipid (mycolic acid) content in the cell wall of Mycobacteria, such as Mycobacterium bovis, rendering them resistant to decolorization by acid-alcohol.
Thus, B matches with I.
- C. Dien's staining: This specialized staining technique is uniquely used to stain colonies of wall-less bacteria, Mycoplasma spp, helping to visualize their characteristic "fried-egg" colony morphology in agar media.
Thus, C matches with IV.
- D. Albert's Staining: This histochemical method is used to stain metachromatic (volutin/polyphosphate) granules of Corynebacterium diphtheriae and related diphtheroids, turning the granules bluish-black against a light green bacillary body.
Thus, D matches with II.

Step 3: Final Answer:
The matching sequence is A-III, B-I, C-IV, D-II, which is option (B).