Question

Match List I with List II 

 

• A. A-II, B-I, C-III, D-IV
• B. A-IV, B-III, C-II, D-I
• C. A-IV, B-II, C-I, D-III
• D. A-III, B-II, C-I, D-IV

Hint:

Memorize the high-yielding pathology dye pairings:
- PAS $\rightarrow$ Glycogen Fungi
- Congo Red $\rightarrow$ Amyloid
- Prussian Blue $\rightarrow$ Ferric Iron Hemosiderin
- Toluidine Blue $\rightarrow$ Mast Cells Heparin

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Histochemistry involves using specific stains to identify chemical constituents, organelles, or abnormal cellular deposits in tissue sections.

Step 2: Detailed Explanation:
Let us match each staining technique with its primary diagnostic target:
- A. Periodic acid-Schiff (PAS): Periodic acid oxidizes adjacent glycol groups to form aldehydes, which then react with Schiff reagent to form a magenta-colored product.
This is the standard stain for identifying carbohydrates like glycogen and glycoproteins.
Thus, A matches with IV.
- B. Congo red: Congo red binds to the beta-pleated sheet configurations of amyloid deposits, exhibiting red-pink staining under light microscopy and apple-green birefringence under polarized light.
Thus, B matches with III.
- C. Prussian Blue: The Perl's Prussian Blue staining technique uses potassium ferrocyanide and hydrochloric acid to react with ferric iron, forming an insoluble blue compound.
This detects intracellular and extracellular iron deposits (hemosiderin).
Thus, C matches with II.
- D. Toluidine Blue: Toluidine blue is a basic metachromatic dye that stains sulfated glycosaminoglycans inside mast cell granules purple-red, creating a distinct contrast against the blue background of other tissues.
Thus, D matches with I.

Step 3: Final Answer:
The exact sequence is A-IV, B-III, C-II, D-I, corresponding to option (B).