Question

Match List I with List II 

Choose the correct answer from the options given below: 
 

• A. A - III, B - IV, C - I, D - II
• B. A - II, B - III, C - I, D - IV
• C. A - II, B - III, C - IV, D - I
• D. A - III, B - II, C - IV, D - I

Hint:

Endospore = Dipicolinic acid (DPA).
Autoclave = High Pressure + High Temperature ($121^{\circ}C$).
Mycoplasma = No Cell Wall (Mollicutes).

Answer 1

The Correct Option is B

Step 1: Understanding the Question:
The question requires matching biological compounds, groups of organisms, and laboratory sterilization techniques with their specific characteristics or tools.

Step 2: Detailed Explanation:

• Dipicolinic Acid (A-II): This chemical compound is found in high concentrations ($10-15\%$ of dry weight) in bacterial endospores. It complexes with calcium ions to provide heat resistance and stabilize the proteins and DNA within the dormant spore.

• Mycoplasmas (B-III): Mycoplasmas are the most well-known members of the class Mollicutes. These are unique bacteria that completely lack a cell wall, making them pleomorphic and naturally resistant to antibiotics that target cell wall synthesis.

• Heat Labile Liquids (C-I): Substances like vitamins, hormones, and some antibiotics are destroyed by heat. To sterilize them, they are passed through bacteriological filters with pores small enough to trap microbes but large enough to let the liquid through.

• Steam under Pressure (D-IV): This is the principle behind the autoclave. By increasing the pressure to $15$ psi, the temperature of steam can be raised to $121^{\circ}C$, which is sufficient to kill even the most resistant bacterial spores within $15-20$ minutes.
Final Answer:
The correct matching sequence is A-II, B-III, C-I, and D-IV. This is represented by option (B).