Question:
In Haematoxylin and Eosin staining, mucin takes following colour:
In Haematoxylin and Eosin staining, mucin takes following colour:
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H&E Staining Affinities:
Nuclei Mucin Cartilage matrix $\rightarrow$ Basophilic (BLUE, binds Hematoxylin).
Cytoplasm Collagen Muscle $\rightarrow$ Eosinophilic (PINK, binds Eosin).
Nuclei Mucin Cartilage matrix $\rightarrow$ Basophilic (BLUE, binds Hematoxylin).
Cytoplasm Collagen Muscle $\rightarrow$ Eosinophilic (PINK, binds Eosin).
Updated On: Sep 7, 2026
- Blue
- Pink
- Violet
- Yellow
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Histological staining properties: acidic epithelial and connective tissue mucosubstances exhibit basophilic staining with hematoxylin.
Step 2: Detailed Explanation:
In standard Hematoxylin and Eosin (H&E) histotechnology:
- Mucin (mucopolysaccharides glycosaminoglycans synthesized by goblet cells and mucous glands) contains abundant negatively charged acidic sulfate ($-SO_3^-$) and carboxyl ($-COO^-$) groups.
- These polyanionic groups react electrostatically with basic cationic Hematoxylin dye lake, staining pale blue to bluish-grey (basophilic) in routine H&E sections.
- (Special mucin stains include Alcian Blue [bright blue] and Periodic Acid-Schiff [PAS, magenta/pink]).
Step 3: Final Answer:
Thus, in H&E staining mucin takes a Blue colour, corresponding to option (A).
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