In E.Coli cloning vector pBR322, antibiotic resistance genes (\(amp^R\)) indicate which restriction sites?
- Pvul, Pstl
- PvulI, EcoRI
- BamHI, SaII
- EcoRI, HindRIII
The Correct Option is A
Solution and Explanation
pBR322 is a cloning vector that was constructed by Bolivar and Rodriguez. The structure of pBR322 has an origin of replication, restriction enzyme sites, and selectable marker sites. pBR322, have two antibiotic resistance genes, ampicillin and tetracycline. They are used as selectable markers. There are multiple restriction sites. In the tetracycline resistance gene, there are sites for BamHI and SalI. In the ampicillin resistance gene, there are sites for PstI and PvuI. There are other EcoRi, HindIII, and ClaI, that do not lie in any of the antibiotic resistance genes regions. In the ROP region, there is a site for PvuII. Therefore, the correct answer is PstI and PvuI.
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Concepts Used:
Applications of Biotechnology
Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.
For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).
Agriculture
Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.
Medicine
In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.
Gene Therapy
A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.
CRISPR technology
For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.
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