Question:

Given below are two statements:
Statement I: Nitrogenase is a catalytically efficient and oxygen-insensitive enzyme.
Statement II: The reduction of acetylene to ethylene cannot be used for the estimation of nitrogenase activity.
In the light of the above statements, choose the most appropriate answer from the options given below:

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Nitrogenase is oxygen-sensitive (requires anaerobic conditions).
Acetylene reduction assay is the standard method for measuring nitrogenase activity.
  • Both Statement I and Statement II are correct
  • Both Statement I and Statement II are incorrect
  • Statement I is correct but Statement II is incorrect
  • Statement I is incorrect but Statement II is correct
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Question:
Nitrogenase is the enzyme responsible for biological nitrogen fixation.
It is found in nitrogen-fixing bacteria and archaea.

Step 2: Detailed Explanation:

Statement I:
Nitrogenase is oxygen-sensitive, not insensitive.
It is irreversibly damaged by oxygen.
Plants protect nitrogenase through leghemoglobin and specialized cells.
This statement is false.
Statement II:
The reduction of acetylene to ethylene is the standard assay for nitrogenase activity.
It is a reliable and sensitive method for measuring nitrogenase activity.
This statement is false.

Step 3: Final Answer:

Thus, both statements are incorrect, which corresponds to option (B).
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