DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- Yellow bands
- Bright orange bands
- Dark red bands
- Bright blue bands
The Correct Option is B
Solution and Explanation
So, the correct option is (B): Bright orange bands.
Top Questions on DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Complete the flow chart on central dogma.
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in NEET exam
- A thin flat circular disc of radius 4.5 cm is placed gently over the surface of water. If surface tension of water is 0.07 Nm-1, then the excess force required to take it away from the surface is :
- NEET (UG) - 2024
- Surface tension
- Which of the following are required for the dark reaction of photosynthesis?
A. Light
B. Chlorophyll
C. CO2
D. ATP
E. NADPH
Choose the correct answer from the options given below:- NEET (UG) - 2024
- Where Does Photosynthesis Take Place?
- If \(x=5\sin(\pi i+\frac{\pi}{3})m\) represents the motion of a particle executing simple harmonic motion, the amplitude and time period of motion, respectively, are :
- NEET (UG) - 2024
- simple harmonic motion
- The highest number of helium atoms is in
- NEET (UG) - 2024
- Electronic configuration of atoms and ions
- \(^{290}_{82}X\stackrel{\alpha}{\rightarrow}Y\stackrel{e^+}{\rightarrow}Z\stackrel{\beta^-}{\rightarrow}P\stackrel{e^-}{\rightarrow}Q\)
In the nuclear emission stated above, the mass number and atomic number of the product Q respectively, are :- NEET (UG) - 2024
- Nuclear physics
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.
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