DNA finger printing technique was first developed by________
- Schleiden and Schwan
- Edwards and Steptoe
- Jeffreys, Wilson and Thien
- Boysen and Jensen
The Correct Option is C
Solution and Explanation
Top Questions on DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
- DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in KCET exam
- If \(\lim\limits_{x \rightarrow 0} \frac{\sin(2+x)-\sin(2-x)}{x}\)= A cos B, then the values of A and B respectively are
- KCET - 2023
- limits of trigonometric functions
- The Curie temperatures of Cobalt and iron are 1400K and 1000K respectively. At T = 1600K , the ratio of magnetic susceptibility of Cobalt to that of iron is
- KCET - 2023
- Magnetism and matter
- A particle is in uniform circular motion. Related to one complete revolution of the particle, which among the stataments is incorrect ?
- KCET - 2023
- Uniform Circular Motion
- The modulus of the complex number \(\frac{(1+i)^2(1+3i)}{(2-6i)(2-2i)}\) is
- KCET - 2023
- complex numbers
- The energy gap of an LED is 2.4 eV. When the LED is switched ‘ON’, the momentum of the emitted photons is
- KCET - 2023
- Semiconductor electronics: materials, devices and simple circuits
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.
SUBSCRIBE TO OUR NEWS LETTER
