Question

Detection of plant parasitic phytoplasma can be achieved through
A. Polymerase chain reaction B. Enzyme-linked immunosorbent assay C. Chromatography D. Restriction Fragment Length Polymorphism
Choose the correct answer from the options given below:

• A. (A), (B) and (C) only.
• B. (B), (C) and (D) only.
• C. (A), (C) and (D) only.
• D. (A), (B) and (D) only.

Hint:

Consider which of these are genuine molecular/serological detection techniques.

Answer 1

The Correct Option is D

Phytoplasmas are unculturable, wall-less bacteria that live inside plant phloem tissue, and because of that, they are usually detected using molecular and immunological techniques rather than direct culturing: PCR to amplify diagnostic DNA sequences, ELISA for antigen-antibody based detection, and RFLP to tell different strains apart after amplification.
Chromatography, which separates chemicals based on their physical or chemical properties, isn't a standard or practical way to diagnose phytoplasma infection.
That makes (A), (B) and (D) correct, matching option 4.