Step 1: Understanding the Concept:
Cervical mucus arborization (ferning): high circulating estrogen at estrus increases sodium chloride and mucin electrolyte concentrations, forming diagnostic palm-leaf crystalline fern patterns.
Step 2: Detailed Explanation:
Mechanism of Cervical Mucus Ferning (Arborization Pattern):
- During Estrus (Heat) (C only), peak preovulatory Estrogen levels stimulate the cervical and vaginal goblet cells to secrete copious, thin, watery, elastic mucus with high concentrations of sodium chloride ($\text{NaCl}$) and mucin.
- When a thin smear of estrous mucus is air-dried on a glass slide, the electrolytes crystallize in a pathognomonic palm-leaf fern-leaf arborization pattern visible under low-power microscopy.
- Under progesterone dominance (metestrus/diestrus), ferning is completely abolished (cellular amorphous smear).
Step 3: Final Answer:
Thus, fern pattern crystallization occurs during C only (Estrus), matching option (C).