Question:

Brucella abortus Indian Biovar(s) has following characteristics:
A. It is modified Z N staining negative
B. It is grown under 5 - 10% CO2 concentration
C. It is urease positive
D. It is non-H2S producer
E. Thayer Martin medium does not show its growth
Choose the correct answer from the options given below:

Show Hint

Brucella abortus Diagnostics: Capnophilic ($5-10\%\text{ CO}_2$), Modified ZN (Stamp's) POSITIVE (Red coccobacilli), Rapidly UREASE POSITIVE.
  • A and D only
  • A and E only
  • B and C only
  • D and E only
Show Solution
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The Correct Option is C

Solution and Explanation


Step 1: Understanding the Concept:

Brucella phenotypic biotyping criteria include carbon dioxide requirement, hydrogen sulfide production, dye tolerance, and rapid urease activity.

Step 2: Detailed Explanation:

Physiological and biochemical characteristics of Brucella abortus (specifically Indian biovars, predominantly Biovar 1 and Biovar 3):
1. CO2 Requirement (B): Primary isolation of B. abortus is capnophilic, strictly requiring 5% to 10% $\text{CO_2$ concentration for growth.
2. Urease Activity (C): It is strongly Urease-positive (hydrolyzes Christensen's urea broth within minutes to hours).
3. Staining: It is obligately Modified Ziehl-Neelsen (Stamp's staining) positive (stains bright red against blue background due to partially acid-fast cell wall), ruling out A.
4. H2S Production: Indian biovars 1 and 3 are $\text{H}_2\text{S}$ positive.
Thus, characteristics B and C are correct.

Step 3: Final Answer:

Hence, the correct characteristics are B and C only, matching option (C).
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