Question:

Before freezing the egg yolk, it should be treated with which of the following enzymes to prevent gel formation after thawing?

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Egg yolk gelation during freezing is prevented by adding $10\%$ sugar/salt or mild proteolytic enzyme treatment.
  • Amylolytic
  • Lipolytic
  • Proteolytic
  • Pectinolytic
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The Correct Option is C

Solution and Explanation

Step 1: Understanding the Concept:
Freezing of egg yolk causes irreversible physical destabilization of low-density lipoproteins (LDL).

Step 2: Detailed Explanation:

When egg yolk is frozen below $-6^\circ\text{C}$ and thawed, it undergoes gelation (gel formation), losing fluidity and becoming rubbery and pasty due to aggregation of LDL apoproteins.
Mild pre-treatment with proteolytic enzymes (such as papain or trypsin) partially cleaves surface proteins, or alternatively adding salt/sugar, prevents lipoprotein aggregation and maintains fluidity upon thawing.

Step 3: Final Answer:

Hence, egg yolk should be treated with proteolytic enzymes to prevent freeze gelation.
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