Question:

Bactofugation is a process which is used to separate

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Centrifugal Dairy Processes:
BACTOFUGATION = Removes BACTERIA AND SPORES (due to higher density $1.2\text{ g/cm}^3$).
Clarification = Removes sediment/leukocytes.
Separation = Separates milk fat (cream) from skim milk.
  • Microorganisms from milk
  • Fat from milk
  • Whey proteins in milk
  • Minerals from milk
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The Correct Option is A

Solution and Explanation

Step 1: Understanding the Concept:
Centrifugal clarification and microbial separation: Bactofugation utilizes high centrifugal force (up to 7,000--9,000 g) in hermetic disc stack centrifuges to separate dense bacterial vegetative cells and anaerobic spores ($ ho pprox 1.12 - 1.30 ext{ g/cm}^3$) from lighter milk plasma ($ ho pprox 1.034 ext{ g/cm}^3$).
Key Formula or Approach:
\[ \text{Centrifugal Force } (F_c) = m \cdot r \cdot \omega^2 \quad \implies \quad \rho_{\text{Bacteria / Spores}} (1.15 - 1.30\text{ g/cm}^3) > \rho_{\text{Milk Plasma}} (1.034\text{ g/cm}^3) \]

Step 2: Detailed Explanation:

In dairy processing and milk clarification technology:
- Bactofugation (A) is a specialized continuous centrifugal separation process developed specifically for removing Microorganisms (vegetative bacteria and bacterial endospores) from milk:
1. Bacterial cells and heat-resistant anaerobic endospores (such as Clostridium tyrobutyricum and Bacillus cereus) have a significantly higher mass density ($1.12\text{ to } 1.30\text{ g/cm}^3$) than milk serum ($1.034\text{ g/cm}^3$) and fat globules ($0.93\text{ g/cm}^3$).
2. Operating at high g-force ($7,000 - 9,000\times g$) and temperature ($55^\circ - 65^\circ ext{C}$), bactofuges achieve a $98\% - 99.5\%$ reduction in bacterial spores and $80\% - 90\%$ reduction in total bacterial count.
3. It is universally employed in cheese milk preparation to prevent late blowing defect in Gouda/Swiss cheese and in ESL (Extended Shelf Life) fluid milk production.

Step 3: Final Answer:

Therefore, Bactofugation is used to separate Microorganisms from milk, corresponding to option (A).
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