Question

Assertion (A): The use of chitinase enzyme is necessary for the separation of DNA from yeast cells but not in the case of Spirogyra.
Reason (R): Fungal cell wall is made up of fungal chitin.

• A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of the Assertion (A).
• B. Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of the Assertion (A).
• C. Assertion (A) is true, but Reason (R) is false.
• D. Assertion (A) is false, but Reason (R) is true.

Hint:

Chitinase is crucial for the breakdown of chitin in fungal cell walls during DNA extraction from fungi. However, it is not required for organisms like Spirogyra that have a cellulose-based cell wall.

Answer 1

The Correct Option is B

Step 1: Understanding the use of chitinase enzyme.
The chitinase enzyme is used to break down chitin, which is found in the cell walls of fungi, including yeast. It is important for isolating DNA from yeast cells. However, Spirogyra, which is an algae, has a different cell wall composition (made up of cellulose), not chitin. Therefore, chitinase is not required for DNA extraction from Spirogyra.

Step 2: Understanding fungal cell walls.
Fungal cell walls are made of chitin, a biopolymer. This explains why chitinase is essential for working with fungi but not with algae like Spirogyra.

Step 3: Conclusion.
While both the assertion and reason are true, the reason does not explain the assertion because the necessity of chitinase for DNA extraction from yeast is not directly related to the fact that fungal cell walls are made of chitin. The correct answer is (B).
Final Answer: (B) Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of the Assertion (A).