A course of three periods of boiling at 100 °C for 30 min. at daily intervals is called
Show Hint
- Pasteurization
- Tyndallization
- Dry heat sterilization
- Incineration
The Correct Option is B
Solution and Explanation
Fractional intermittent heat sterilization is employed for thermolabile culture media destroyed by pressurized autoclaving.
Step 2: Detailed Explanation:
Tyndallization (fractional steam sterilization, developed by John Tyndall) involves exposing the medium to flowing steam at $100^\circ\text{C}$ for 30 minutes on three successive days with intermediate incubation at $30\text{--}37^\circ\text{C}$.
The first heating kills vegetative bacterial cells; during the 24-hour interval, heat-activated resistant endospores germinate into sensitive vegetative cells, which are eliminated during subsequent heatings.
Step 3: Final Answer:
Therefore, this three-period boiling process is called Tyndallization.
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