List of top Biochemistry Questions on UV-visible and fluorescence spectroscopy asked in GATE XL

Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
A fluorescence microscope with an objective lens of numerical aperture (NA) 1.5 is used with light of wavelength (λ) 600 nanometers. The lateral resolution limit of this microscope rounded off to the nearest integer, is______nanometers.
Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
A forty-times diluted sample of ssRNA gave an \( A_{260} \) of 0.01. The concentration of the ssRNA before the dilution in \(\mu g/mL\) was ______ (correct to the nearest integer).
The absorbance of a \( 5 \times 10^{-4} \, M \) solution of tyrosine at 280 nm wavelength is 0.75. The path length of the cuvette is 1 cm. The molar absorption coefficient at the given wavelength in \( \text{M}^{-1} \text{cm}^{-1} \), correct to the nearest integer, is ______.