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List of top Biochemistry Questions on Biochemical separation techniques asked in GATE XL

Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II? \[ \begin{array}{|c|c|} \hline \textbf{Column I} & \textbf{Column II} \\ \hline P. \, \text{Solubility} & iii. \, \text{Salting out} \\ \hline Q. \, \text{Ionic charge} & iv. \, \text{Isoelectric focusing} \\ \hline R. \, \text{Polarity} & i. \, \text{Reverse phase chromatography} \\ \hline S. \, \text{Molecular size} & v. \, \text{Gel electrophoresis} \\ \hline \end{array} \]
  • GATE XL - 2024
  • GATE XL
  • Biochemistry
  • Biochemical separation techniques
Purification of 6×His-tagged protein using Ni-NTA column is an example of
  • GATE XL - 2022
  • GATE XL
  • Biochemistry
  • Biochemical separation techniques
A dye was allowed to undergo migration on a chromatographic paper using a solvent. The dye, and the solvent-front migrated 5 and 20 cm, respectively, from the point of origin. The retention factor (rounded off to two places decimal) for the dye is ________.
  • GATE XL - 2022
  • GATE XL
  • Biochemistry
  • Biochemical separation techniques