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List of top Biochemistry Questions on Bioenergetics and metabolism asked in GATE XL

2-Deoxyglucose (2-DG) inhibits the proliferation of cells and hence finds use as an anti-cancer agent. It is also used in COVID therapy, where it blocks hyperproliferation of virus-infected cells. Mechanistically, 2-DG blocks glycolysis by inhibiting the activities of which of the following enzyme/s?
  • GATE XL - 2022
  • GATE XL
  • Biochemistry
  • Bioenergetics and metabolism
The standard free energy changes for conversion of phosphoenol pyruvate (PEP) to pyruvate, and ATP synthesis are shown below. \[ \text{PEP} + \text{H}_2\text{O} \rightleftharpoons \text{pyruvate} + \text{P}_i \quad \Delta G^\circ = -61.9 \, \text{kJ/mol} \] \[ \text{ADP} + \text{P}_i \rightleftharpoons \text{ATP} + \text{H}_2\text{O} \quad \Delta G^\circ = 30.5 \, \text{kJ/mol} \] The starting concentrations of PEP, ADP, pyruvate, and ATP are 25, 25, 50, and 50 mM, respectively. The value of the universal gas constant (R) is 8.315 J·mol\(^{-1}\)·K\(^{-1}\). The actual free energy change in kJ·mol\(^{-1}\) for the reaction \[ \text{PEP} + \text{ADP} \rightarrow \text{pyruvate} + \text{ATP} \] carried out at 37°C will be ________ (rounded off to one place of decimal).
  • GATE XL - 2022
  • GATE XL
  • Biochemistry
  • Bioenergetics and metabolism
Which one of the following enzymes is located in the outer mitochondrial membrane?
  • GATE XL - 2022
  • GATE XL
  • Biochemistry
  • Bioenergetics and metabolism