List of top General Biology Questions on Enzyme kinetics: Michaelis-Menten equation asked in GATE BT

The catalytic efficiency of an enzyme following Michaelis-Menten kinetics is defined by

You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is 1800 \(\mu\)moles L^-1 min^-1 and the total concentration of the enzyme in the reaction mixture is 1.5 \(\mu\)M, then the turnover number of the enzyme is s^-1. (answer in integer)

For enzyme \(\alpha\)-amylase with \(K_m = 0.005\,M\), the substrate concentration for one-fourth of \(V_{\max}\) (rounded to two decimals, mM) is \(\underline{\hspace{1cm}}\).