List of top Biotechnology Tools and Techniques Questions on Molecular Biology asked in GAT-B

The distance between each turn in the helical strand of DNA is
Which of the following DNA, in its double-stranded form, will have the highest \(T_m\)?
Which of the following is generally NOT a step in PCR?
Which of the following could be a recognition site of a Type II restriction endonuclease?
During replication, DNA polymerases distinguish between ribonucleoside and deoxyribonucleoside triphosphates (rNTPs and dNTPs) while synthesizing new DNA strand. This is because:
Regulation of lac operon is negative as:
Which of the following can be a restriction site for type II restriction enzyme?
A double-stranded genomic DNA has 40% (A+T) content. What is the average size (base pairs) of the fragments generated by digestion with the restriction enzyme HaeIII (GGCC)?
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: Attenuation of the trp operon is possible in bacteria.
Reason R: Transcription and translation are coupled.
The following diagram represents a restriction enzyme site:
Statement I: After digestion cohesive ends will be formed.
Statement II: These cohesive ends will have phosphate groups at 3' and 5' ends.
Lac repressor of E. coli contains ________ DNA binding motifs.
Which protein is required to seal a nick during Okazaki fragment maturation?
Self splicing introns are an example of:
Given below are two statements about hnRNA processing:
Statement I: Capping occurs by addition of methyl adenosine triphosphate to the 5' end.
Statement II: During tailing, about 200-300 adenylate residues are added to its 3' end.
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: A linear DNA molecule containing three recognition sites for a restriction enzyme will generate multiple DNA fragments on partial digestion.
Reason R: Partial digestion by restriction enzymes leads to a heterogeneous population of DNA fragments.
Given below are two statements:
Statement I: The fragments of DNA generated after cleavage with a restriction enzyme have a phosphate group on their 3' ends and a -OH group on their 5' ends.
Statement II: Cohesive ends generated by restriction digestion reanneal with complementary DNA fragment.
Which one of the following techniques can be used to determine the copy number of a gene?
Which enzyme catalyzes strand separation at the replication fork?
Which one of the following statements correctly describes type IIP restriction enzymes?
A. They cut nucleotides only from the ends of the DNA.
B. They act on only palindromic nucleotide sequences in the DNA.
C. They recognize only GC rich sequences.
D. They recognize only AT rich sequences.
Match the enzymes in the List I to their respective functions in the List II.
The restriction endonuclease which cleaves the given sequence at the indicated site is: $$(5') G \downarrow GATCC (3')$$ $$(3') CCTAG \uparrow G (5')$$
EcoR1 enzyme recognizes the sequence GAATTC. A stretch of linear DNA with six sites will give rise to
A 5200-bp vector contains two BamHI sites 450-bp apart. A 1200-bp gene "X" with BamHI sites at ends is cloned after complete digestion. Size of the resultant plasmid?
Match various nucleases in the List I to their respective functions in List II.
Nick translation is carried out by