List of top Biotechnology Questions on Biochemistry asked in GAT-B

Match List I with List II and choose the correct answer.

Information about the isoelectric point will be useful in the purification of a protein by which of the following chromatography techniques?

The approximate length of the open reading frame coding for a 55 kDa bacterial protein will be

Immobilization of enzymes:
A. increases the specificity of the enzyme in batch reaction
B. leads to an increase in the apparent $K_{m}$
C. makes it unsuitable for its use in a continuous reactor system
D. decrease the operational cost of the industrial process

Match items in List I with items in List II
List I (Enzyme): A. Cytochrome oxidase, B. DNA polymerase, C. Nitrate reductase, D. Urease
List II (Cofactor): I. Molybdenum, II. Nickel, III. Magnesium, IV. Copper

In a batch experiment with cells following Monod growth kinetics, the initial substrate concentration is doubled from $S_0$ to $2S_0$ and it is observed that the initial doubling time in the log phase hardly changes. This tells us that

In an ideal Continuous Stirred Tank Reactor (CSTR) at steady state