The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel.
The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
The fragments of DNA separate on agarose gel, based on the.
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel.
The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
The fragments of DNA separate on agarose gel, based on the.
Size of fragments
Charge of each fragment
Colour of fragment
Type of DNA
The Correct Option is A
Solution and Explanation
The fragments of DNA separate on agarose gel based on the "Size of fragments."
Top Questions on biotechnology
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Questions Asked in CUET exam
- A molecule X associates in a given solvent as per the following equation:
X ⇌ (X)n
For a given concentration of X, the van’t Hoff factor was found to be 0.80 and the
fraction of associated molecules was 0.3. The correct value of ‘n’ is:- CUET (UG) - 2024
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- A company produces 'x' units of geometry boxes in a day. If the raw material of one geometry box costs ₹2 more than the square of the number of boxes produced in a day, the cost of transportation is half the number of boxes produced in a day, and the cost incurred on storage is ₹150 per day. The marginal cost (in ₹) when 70 geometry boxes are produced in a day is:
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Concepts Used:
Applications of Biotechnology
Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.
For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).
Agriculture
Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.
Medicine
In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.
Gene Therapy
A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.
CRISPR technology
For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.
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