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List of top Biology Questions on biotechnology asked in NEET (UG)

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

Which of the following is not a cloning vector?
Which of the following is not a desirable feature of a cloning vector?

Statements related to human insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin?

  1. Pro-hormone insulin contain extra stretch of C-peptide
  2. A peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating a disulphide bond between them.
  3. Insulin used for treating Diabetes was extracted from Cattles and Pigs.
  4. Pro-hormone insulin needs to be processed for converting into a mature and functional hormone.
  5. Some patients develop allergic reactions to the foreign insulin.

Choose the most appropriate answer from the options given below:

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:
In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?
Given below are two statements:
Statement I: Restriction endonucleases recognise specific sequences to cut DNA known as palindromic nucleotide sequences.
Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below: 
Which one of the following statements is not true regarding gel electrophoresis technique?
The Adenosine deaminase deficiency results into
Match List - I with List - II.
                 List-I         List-II
(a)Aspergillus niger(i)Acetic Acid
(b)Acetobacter aceti(ii)Lactic Acid
(c)Clostridium butylicum(iii)Citric Acid
(d)Lactobacillus(iv)Butyric Acid 
 Choose the correct answer from the options given below.
 

In spite of interspecific competition in nature, which mechanism the competing species might have evolved for their survival?

Which of the following stages of meiosis involves the division of the centromere?

During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out :

Match List - I with List - II. 

List - I

List - II

(a)Lenticels(i)Phellogen
(b)Cork cambium(ii)Suberin deposition
(c)Exchange of gases(iii)Exchange of gases
(d)Cork(iv)Phelloderm 

 Choose the correct answer from the options given below

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because:
With regard to insulin choose correct options. 
(a) C-peptide is not present in mature insulin. 
(b) The insulin produced by rDNA technology has C-peptide. 
(c) The pro-insulin has C-peptide. 
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

Which of the following is an incorrect statement ?

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain
Identify the wrong statement with regard to Restriction Enzymes.
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:

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