List of top Biology Questions on Biotechnology asked in Common University Entrance Test

Which of the following products is not developed by the use of biotechnology?

Match List-I with List-II:

List-I(Microbes)	List-II (Acid Produced)
(A) Aspergillus	(I) Lactic acid
(B) Acetobacter	(II) Butyric acid
(C) Clostridium	(III) Acetic acid
(D) Lactobacillus	(IV) Citric acid

The thermostable the enzyme used in PCR is

Identify the correct statements regarding features of a vector.
A. They should have ability to replicate within the host
B. They should have ‘ori’ sites that start replication
C. Its origin should not support high copy number
D. They should have more than one recognition sites for restriction enzymes
Choose the correct answer from the options given below :

Arrange the following steps of PCR in correct sequence.
(A) Temperature induced denaturation of double stranded DNA. 
(B) Isolation of gene of interest 
(C) Amplification of gene by using Taq polymerase 
(D) Primer annealing on the separated templates 
Choose the correct answer from the options given below:

Match LIST I with LIST II

LIST I		LIST II
A	First Restriction Endonuclease	I	GAATTC
B	Disarmed Plasmid	II	Selectable markers
C	Antibiotic resistant genes	III	Hind II
D	EcoRI	IV	Vector

Choose the correct answer from the options given below

Which of the following device is used to culture large volumes of living cells to get products on large scale?